I'm having trouble doing the STATS for my dissertation. So far I have ran two-way Anova's for some results and I had to use this by doing the following...

Normal distribution?

Levene's Test?

Mauchleys test of sphericity?

2-way ANOVA

Independent t-test

I have no idea what any of these mean but i done them anyway with a little help from my tutor.

But the thing is, i am now onto a set of results with only 2 groups/variables or whatever you call them instead of three i.e. time periods.

So when i run the above tests I can a p value of ''.'' for mauchleys test of sphericty and the other eplisom values are ''1.000''

is this the wrong test? should i be using something else like a 1 way anova or manova?

I'm trying to use youtube tutorials but i am lost.

Any help would be much appreciated, please find the attached picture. you will see that i am assessing leucine v a control over 2 time periods, post 24hrs and 48hrs.

Many Thanks