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Thread: chi square problem.

  1. #1
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    chi square problem.




    hi all:

    I tried google and the search function on this forum with no luck. I did the following experiment:

    Differentiate a stem cell into four different types of cell, let's call them type A, B, C and D.

    The control group usually has a 25% A, 25% B, 25% C and 25% D distribution.
    The treatment group usually has 80% A, 5% B, 5% C and 10% D distribution.

    However, the word "usually" is my problem. Each group (control and treatment) is done in triplicates, meaning I have 3 sets of distribution data for eatch group. The 3 sets of distribution ratio within each group are all similar to the ones shown above however they ARE different. I suppose if I carry out Chi-square analysis, assuming it is the right analysis, I need to take into account the mean and the standard deviation for both the control and the treatment group. But I just can't seem to find the right formulae. Every site I've been to gives me the following formulae:



    where Oi = an observed frequency (ie count) for the ith bin
    Ei = an expected (theoretical) frequency for the ith bin

    Thanks in advance for your help.

  2. #2
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    In other words, if I have something like:

    The control group has a 253% A, 253% B, 253% C and 253% D distribution.
    The treatment group has a 804% A, 104% B, 54% C and 54% D distribution.

    How do I carry out chi-square analysis while taking the standard deviation into account?

  3. #3
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    one more question, if I have two different treatment in addition to the control, is there anything like a chi-square/ANOVA combo analysis for this?

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