What method are you using to analyze the data? For t-test you can have unequal sample sizes. This was covered here in the past. http://www.talkstats.com/showthread....l-sample-sizes
From memory in certain cases of ANOVA not having the same subgroup n can complicate analysis of the data, but the real leason here is that your question depends on which method you are used to analyze the data with.





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