Originally Posted by

**BGM**
Not sure if I understand the terminologies correctly.

total amount of cells filtered - is it just the total number of cells? (5 in your example)

Suppose you have calculated that on average 32.805% of the wells (out of 10) contain a single cell only (I haven't check any calculations). Since for each singly-filtered wells contain a single cell only, the number of singly-filtered cells is just equal to the number of the singly-filtered wells, and thus on average the number is 32.805% * 10, and the proportion of cells which is singly-filtered is 32.805% * 10 / 6.