If im looking at current method vs new method for 8 specimens -

1)Semis vs Semis
2) Ultras vs Ultras

and instead i look at statistics to score individual parameters for each eg.

Semi thin sections- Score 1-3 -


Quality of staining Strong or weak? Excellent to very poor

Preservation of staining Artefacts? Scores?

Nuclei/cell preservation




Ultra thin sections Scores 1-5

Artefact Staining contamination, dust, creases, scores?

Contrast Black/white grey scale

Organelles recognisable? Mitochondria, lysosomes, nuclei, membranes, inclusions.

Resolution Good/grainy background?




Eg for each specimen ( both curent+new method) the ultra thin section will get 4 scores from the parameters

eg

Ultra thin section scoring

Specimen 1

Current method

ARTEFACT 2
CONTRAST 3
ORGANELLES 4
RESOLUTION 3


Specimen 1

New method

ARTEFACT 2
CONTRAST 3
ORGANELLES 4
RESOLUTION 3

How do i test the null hypothesis of no difference?
Can I do a paired samples test?


Appreciate any help, as not sure how I can link the two together at the moment