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Thread: Significant differences in significant differances

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    Significant differences in significant differances




    Hello forum Gurus,
    I have tried search for a problem similar to mine in these forum to no avail. So here goes. I have 4 groups (A,B,C,D) with 4 biological replicates each. I have calculated the fold change in gene expression between Group A and Group B as well as between Group C and D and if that fold change ratio is significant or not. Now I want to determine if there is a significant difference in the A/B ratio and the C/D ratio and I am stuck on how to do this. Any suggestions are welcome and thanks ahead of time.

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    Re: Significant differences in significant differances

    What type of data is this? (Microarray? RNA-seq?)
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    Re: Significant differences in significant differances

    Its RNA-seq data analyzed via cuffdiff.

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    Re: Significant differences in significant differances


    I am thinking that where the data came from isn't relevant as much as how I would like to analyze it. Most of the time the expression data is normally distributed if that helps with suggestions

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