Dear Biostatistician,

I have done an AMOVA analysis on mtDNA sequences, partitioned on 14 populations (with a variable number of individuals in each populations), and I insert all the population in the same group.

From the AMOVA analysis I have obtained significant value for Fst among populations, but when i try to compute pairwise Fst between the populations I obtaine only 3 significant pair and the other are not significant.

Is it possible? May the problem is that i have to redefine my populations?

Thank you