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Thread: Nested design

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    Nested design




    I am conducting a study investigating the effects of different media treatments on protein expression in cells. I have four levels of media treatment which are crossed with valproic acid or no valproic acid (ie a 4x2 factorial design).

    The question I have is with regards to nesting. Each observation (in the form of a picture) is one of a set of 4 taken from a single well. These are replicated in 2 different plates, which are from one of 3 subjects in either of two groups. Should I be nesting these variables in my ANOVA, or is the effect of this irrelevant?

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    Re: Nested design


    hi,
    I would use a nested design because otherwise the analysis would treat the data points as independent samples which they are not.

    Regards
    rogojel

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