Hello,

I want to test effect of PH on larval development. I would like to know what is the best experimental design and statistical analysis.

We can only use 3 compartments, each one with a different ph. In each compartment, larvae will grow in 4 differents cylinder.

Each day, we will take off severals larvae in each cylinder, measured larvae and count proportion of dead larvae.

Can I then analyse proportion of dead larvae per day with a GLMM with cylinder in random factor (repeted measures ?) ?

Can I "use" different parents to produce larvae of cylinders and analyse a kind of genetic effect ?
What should be my model in this case ?


Thank you.