# Thread: Hopefully Simple Question: Two Way ANOVA or One-Way Repeated Measures?

1. ## Hopefully Simple Question: Two Way ANOVA or One-Way Repeated Measures?

Hi all, I'm new here but have been wrestling with a statistical question for a while now, getting different answers depending who I ask. I'm really trying to increase my statistics know-how, but it's tough. Alright, that's my disclaimer, here is my real question:

I work with mouse muscle cross sectional area (CSA). Basically, mice are on drug trials, we section their muscle, and measure the CSA for 300-500 individual fibers from each muscle. Plotting on a histogram, these are generally slightly skewed right, though it can vary. We want to compare changes in CSA between the mice in the drug and control group. I've been mostly told 2 things about how to do this - do a one way ANOVA with repeated measures to compare drug vs control, treating the CSA of each individual muscle fiber as a separate repeated measure. Alternately, I've been told to do a two way ANOVA with one factor being drug vs control while the other is which animal is which, to look at variability both within groups and between groups. Inherently both seem to make sense to me in some ways but not others. Any help/clarity would be very greatly appreciated!

Thanks.

2. ## Re: Hopefully Simple Question: Two Way ANOVA or One-Way Repeated Measures?

Couldn't you aggegate each individual's 300-500 measures
and use this aggregated variable as dependent variable in

With kind regards

K.

3. ## Re: Hopefully Simple Question: Two Way ANOVA or One-Way Repeated Measures?

Thanks! The idea of aggregating the values makes sense to me - would I theoretically then be able to run a One-way ANOVA looking at drug versus control? Sorry for such simple questions, this is like learning a new language for me - a fascinating one, but one that can easily cause some headaches

Edit to also add: my plan is to try to figure this out in R, which I am very, very slowly learning... Not sure if that will give anyone any further insight before I head into the R subforum. This place is such an awesome resource!

4. ## Re: Hopefully Simple Question: Two Way ANOVA or One-Way Repeated Measures?

The idea of aggregating the values makes sense to me - would I theoretically then be able to run a One-way ANOVA looking at drug versus control?
You could then perform a t-test, or a Mann-Whitney U-test (if total
number of mice is low and/or measurements are heavily skewed).

With kind regards

K.

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