Hi everyone,

I am currently working on my MSc thesis and have talked myself around in circles and have gotten completely lost.

I am trying to determine the variability seen within two repeats of my assay (antibody titration).

I have multiple concentrations that I have repeated twice giving titration score.

Should I be using a one-way ANOVA or t-test?

I am confused with what I am actually analysing - the difference between the two repeats of each concentration or the differences between the means of the two repeats for the multiple concentrations?

You wouldn't believe I have an A-Level in statistics, but it appears after 10 years if you don't use it you loose it.

Any help is much appreciated.