I really need your help.

We have just run a small experiment where we treated some animals with 2 compounds that are potential treatment for a disease which was induced through drinking water. The animals were assessed by a score from 0-3.

n=12 in each group that had the disease induced and only n=5 in the normal animals (no induction of disease or treatment).

We scored the animals at day 7 , 10, 14 and 21.

If I use a 2-way ANOVA on the all the data (from the attached dataset) there are no significant difference between the groups even though the mean values a lot lower in treatment 2 group (type 2 error?).

but if I use the 2way ANOVA on the mean score of each group (also in the dataset lower part), then it gets significant but is this allowed.

I also tried the 1way ANOVA on the mean values, which turns out significant. Is this a better way of describing the data?

I really hope that you can help me out as this is very important

Thank you very much for clarifying this so a regular t-test guy can understand this

View attachment 4155