Comparing data from single samples

drb

New Member
#1
I measured mutations after drug treatment in two different lines (genotype A and B) at 3 different timepoints (1,2,3). Is there a test to see whether A1 is different from A2, A2 is different from A3, and A1 is different from A3? I also want to compare A1 to B1, A2 to B2 and A3 to B3. I do not know what the sampling error is at any time point.

time A B

1 186 256

2 259 313

3 295 325

(I know this is not likely very meaningful but a reviewer is demanding a statistical analysis on this data).
 
#2
Hi, do you measure different individuals forach each Geontype? That would mean that your tabular represents mean values? Without any grasp of the variance of each measurement I think you can hardly make any statistics....
 

drb

New Member
#3
Hi, do you measure different individuals forach each Geontype? That would mean that your tabular represents mean values?
It is a pool of 10 individuals, but they are not individually bar-coded, so the value is a hypothetical mean (total mutations/10 individuals) but not a measured one. (Hypothetical in that it assumes equal representation of each individual in the pool).

Without any grasp of the variance of each measurement I think you can hardly make any statistics....
I agree, which is why I did not provide them in the first place. The only way to assess variance would be to do multiple replicates for each genotype and time point, and at $1000/measurement that ain't gonna happen.