For example: Normal cells have 3 junctions after 120 seconds, 6 junctions after 180 seconds...

The mutated ones form significantly less junctions by the end of two hours, and there is also a bigger time lag before they start forming junctions.

I have data for 5 repeats for each type of cell.

The pattern can clearly be seen by eye and by plotting a graph. However, I need to statistically analyse it. What test should I use to show that there is actually a significant difference?

Thanks