Hi,
I'm a grad student in behavioural neuroscience.
I am looking at individual difference in anxiety in animals.
There are two groups of animals: one anxious and the other non-anxious.
I present then with a potential threat and see if their anxiety levels are reflected on their behaviour.
In the test paradigm, I place a threatening object in front of a cage for a certain time.
The cage is divided into 4 sections: Front, Middle, Back and Furthest back.
I measure proportions of time spent at each location.
For instance, a particular animal spent 20% Front, 40% Middle, 30% Back and 10% Furthest back.
Thus, independent variable is the group; anxious and non-anxious.
Dependent variable is the proportion of time spent at each location.
In order to see if there is any difference between the groups, I am doing a t-test for each location.
But, would it be possible to do a 2-way ANOVA with independent variables: Group (Anxious, Non-anxious) and Location (Front, Middle) and dependent variable: proportion of time spent at each location?
Since the locations are not independent from each other, will this violate the independence of observation?
Thank you,
I'm a grad student in behavioural neuroscience.
I am looking at individual difference in anxiety in animals.
There are two groups of animals: one anxious and the other non-anxious.
I present then with a potential threat and see if their anxiety levels are reflected on their behaviour.
In the test paradigm, I place a threatening object in front of a cage for a certain time.
The cage is divided into 4 sections: Front, Middle, Back and Furthest back.
I measure proportions of time spent at each location.
For instance, a particular animal spent 20% Front, 40% Middle, 30% Back and 10% Furthest back.
Thus, independent variable is the group; anxious and non-anxious.
Dependent variable is the proportion of time spent at each location.
In order to see if there is any difference between the groups, I am doing a t-test for each location.
But, would it be possible to do a 2-way ANOVA with independent variables: Group (Anxious, Non-anxious) and Location (Front, Middle) and dependent variable: proportion of time spent at each location?
Since the locations are not independent from each other, will this violate the independence of observation?
Thank you,