My dissertation research evaluates the effect of a hormone on the epithelium. My data was obtained from a clinical trial with a placebo group and 4 different treatment groups. For each group I have pre and post treatment samples but an unequal number of data points.

I want to compare the pre and post treatment among individuals. The post treatment samples were taken from three different sites of the foreskin, while the pretreatment sample was taken from only one area. Should I use mixed model nested anova for the comparison?

I also want to compare the placebo group against all of the four treatment groups. Initially I thought that I could use the two sample unpaired, unequal variance test. After reading through information on choosing which statistical test to use, I do not think it is the appropriate test.

Any help would be greatly appreciated. Thanks in advance!