Please help!

#1
Admittedly, I am not so good at stats, so forgive me if these seem like stupid questions. I am working on a Ph.D. in cell/molecular biology, studying the effects of a gene mutation. In my experiments, I have a number of variables and I am attempting to discern how I should analyze my data. I vary:

1. Genotype: a cell can have 0, 1, or 2 functional copies of my gene
2. Sex: obviously, male or female
3. Treatment: I treat my cells with two different chemicals at four different concentrations of each alone and in combination, making for 16 different treatments.

So, if I understand correctly, I need to run a 4 way ANOVA (genotype, sex, treatment 1, treatment 2). Is this correct? Also, does anyone have a suggestion of good software to tackle this problem?

Thanks for the insight! :)
 
E

elnaz

Guest
#2
Hello
please , tell me your aim about your data
you should tell me whats your goal for analysis?
then if you want , send your data to me that i analyze it but you should tell me your aim certainly.

Best regards
Elnaz
 
#3
My aims are to determine whether (1) partial or complete mutation in "gene X" alters evoked changes in intracellular calcium homeostasis compared to non-mutant cells, and (2) if the partial/complete lack of gene X will increase a cell's responsiveness to toxic insult compared to non-mutant cells, again by analyzing alterations in evoked cytoplasmic calcium waves.

I appreciate the offer to analyze it, but I would rather do so myself if I can, so I can understand my data better and for my future reference as well.

Thanks in advance for any input.

LabGirl