T-test, linear mixed effect model or third solution?

Hi all,

I'm investigating if there is a difference in volume of a specific brain region when comparing Migraneurs with aura to healthy controls. I would like to adjust for age and the fact that i have twin pairs in the Migraneurs group. I've thought about creating a linear mixed effect model, but I lack experience using these models. Can anybody help me on the way? Would it be possible to solve this problem using simple t-tests?

Thanks a lot!

Simple setup: Migraneurs vs. Healthy controls, adjusted for age, adjusted for twin pairs in the Migraneurs group.

- JD