What is the best statistics test for this questions??

Can somebody help me in my statistical problems?
I have measured heart rate of fish during temperature increased. I have wanted to find temperature break points.

I have three different case which I should be compared together.
1) heart rate in vivo (ekg)
2) heart rate in heart
3) heart rate in myocytes

See figure attachment files.
I want to test that is there differences between these three curves? What is the best and correctly test in this situation?

Thanks a lot,


New Member
What do the error bars represent?

Do you really need to do tests? The data speaks pretty much for itself, right?

Also, coming up with hypotheses after the study has been done is a bit odd. I'd say just leave it at these descriptive data