I have done a lot of linear modeling for my thesis, however one of the unsolved stat problems I have should be a pretty basic issue. I am testing to see if an animal is moving from its nest to the stream by following the shortest path or the decline in slope. I drew a straight distance line, and a line that follows the slope as if water was poured from the nest using ArcGIS. I then measured the distance from each animal's waypoint to both lines. So far I used a studen'ts paired t-test to test the significance between the distance from the slope line and the straight line (acting as a null). One of my reviewers mentioned this may be incorrect as the student's paired t-test is usually used for before and after a treatment, such as administering a drug. It has been awhile since I have ran basic stat test, and I greatly appreciate any feedback and advice. Thanks!