which test should i use?

[aharrygeorge]

Hi, if anybody could help with some Biological statisitics then this would be so great:
I'm trying to analyse the significance of some cell culture results, looking at the difference between cultures. (I have screwy results, so its not a simple analysis unfortunatly)

eg. each culture would be like these, always in increments of .25:
1
1,25
1,0

0
0,5
0,25

1,0
1,25
1,25

ETC..

These can be either values from a day 1 or 2,3,6.

And either 25ml or 50ml

the first value is always: IFN-containing Medium
the second: Non-stimulated Medium
the third: Control Medium

Now I tried putting this into MiniTab, I have no idea what to do, I was suggested to use the General Linear Model, then a test of Equal Variance and a Normality Test (anderson-darling) but am not really sure what or even why I'd choose these tests.

-----
One last question..

I would enter the results into MiniTab as below for example:

data virus ttmt
1 25 1
1,25 25 2
1,0 25 3
0 25 1
0,5 25 2
0,25 50 3
1,0 50 1
1,25 50 2
1,25 50 3

ok, so could i just add another column to show the "day"? eg

day
1
1
1
1
2
2
2


As how would i analyse differences between the days? This, nobody can seem to help me with.

If anybody has any suggestions on methods to apply to such results that would be a real help because I am clueless.

Thanks

 

[bugman]

It sounds like a two-way ANOVA (days and volume as factors) but first, please clarify what you response variable is - is it cell number?

Your normaility and equal variance tests are run to check the assumption of your model.

 

[aharrygeorge]

hi, thanks for your reply,

ok two way anova seems right.

what do you mean by response variable?

thanks again

 

[bugman]

Your response variable is what ever you are measuring. eg. cell number, density survival etc...