We have 6 kinds of material and have 4 group for each material now(please see the attached table)

we cut one tooth to two halves(A and B) and bonded two composite cylinder to each half(cylinder 1 and 2). half A is control group(just following the normal bonding process) and half B is treated group(treated with one of the material first and then following the normal bonding process).That means, In total, we make four cylinder on one tooth.(A1.A2,B1,B2). We test the shear bonding strength after 24h(A1 and B1) and after 1week(A2 and B2)

In general:

A1 = ControlGroup after 24h

A2 = Control Group after 1w

B1 = Experimental Group after 24h

B2 = Experimental Group after 1w

we want to test:

1. if all of the material will have influence on the shear bond strength(bonding effect)

2. how the shear bond strength changes following the storage time(24h and 1w)

I have think about several methods:

1) use the paired t-test to analyze the relationship between A1 and A2, B1 and B2(different time)

use the independent t-test to analyze the relationship between A1 and B1, A2 and B2(with treatment and without treatment)

2) just use one way ANOVA to analyze all the four group per each material. This method can not analyze the difference within different materials.

3）use two-way ANOVA(time and treatment is two factors).(the problem is we can got the result about if "time" or "treatment" has influence, but we can not see the details between A1 and A2, B1 and B2, A1 and B1,A2 and B2)

4) use repeated-measures ANOVA to analyse(time is within-subject factor, treatment is between-subject factor)

5) if we also want to analysis the difference between different solution, maybe we should choose three-way ANONVA.( time, treatment, different materials as three factor, the problem is the same with 3), we can not get the details between A1 and A2, B1 and B2, A1 and B1,A2 and B2)

I really can not decide which method is suitable for my data, I am very appreciated if you can give me some advice. Thank you very much!